Article
Targeted insertions of two exogenous collagen genes into both alleles of their endogenous loci in cultured human cells: the insertions are directed by relatively short fragments containing the promoters and the 5' ends of the genes.
Proceedings of the National Academy of Sciences of the United States of America - 19 Jul 1994
Ganguly A, Smelt S, Mewar R, Fertala A, Sieron A L, Overhauser J, Prockop D J
Abstract excerpt
Previous studies demonstrated that type II procollagen is synthesized by HT-1080 cells that are stably transfected with constructs of the human COL2A1 gene that contain the promoter and 5' end of either the COL2A1 gene or the human COL1A1 gene. Since the host HT-1080 cells were from a human tumor line that synthesizes type IV collagen but not type II or type I procollagen, the results suggested that the...
Topics
- 3T3 Cells
- Alleles
- Animals
- Chromosomes, Human, Pair 12
- Cloning, Molecular
- Collagen
- Cosmids
- DNA
- Humans
- In Situ Hybridization, Fluorescence