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Article

Direct selection for mutations affecting specific splice sites in a hamster dihydrofolate reductase minigene.

Molecular and cellular biology - 1 Jan 1993

Chen I T, Chasin L A

Abstract excerpt

A Chinese hamster cell line containing an extra exon 2 (50 bp) inserted into a single intron of a dihydrofolate reductase (dhfr) minigene was constructed. The extra exon 2 was efficiently spliced into the RNA, resulting in an mRNA that is incapable of coding for the DHFR enzyme. Mutations that de...

Topics

Animals
Cricetinae
Cricetulus
Gene Expression
Genes
Hydrogen Bonding
Mutagenesis
Mutation
Polymerase Chain Reaction
RNA Splicing
RNA, Messenger
Selection, Genetic
Structure-Activity Relationship

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Tetrahydrofolate Dehydrogenase

Source

Open in PubMed Open full text

Identifiers

PMID: 8417332
DOI: 10.1128/mcb.13.1.289-300.1993
PMCID: PMC358908

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8417332-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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