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Article

Direct selection for mutations affecting specific splice sites in a hamster dihydrofolate reductase minigene.

Molecular and cellular biology - 1 Jan 1993

Chen I T, Chasin L A

Abstract excerpt

A Chinese hamster cell line containing an extra exon 2 (50 bp) inserted into a single intron of a dihydrofolate reductase (dhfr) minigene was constructed. The extra exon 2 was efficiently spliced into the RNA, resulting in an mRNA that is incapable of coding for the DHFR enzyme. Mutations that decreased splicing of this extra exon 2 caused it to be skipped and so produced normal dhfr mRNA. In contrast to the...

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Identifiers

PMID: 8417332
DOI: 10.1128/mcb.13.1.289-300.1993
PMCID: PMC358908

Genes

DHFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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