Article
Variation of minisatellites in chemically induced mutagenesis and in gene amplification.
EXS - 1 Jan 1993
Vagnarelli P, Giulotto E, Fattorini P, Mucciolo E, Bensi M, Tessera L, De Carli L
Abstract excerpt
A mutation assay in cultured mammalian cells was developed based on direct analysis of minisatellite DNA. Chinese hamster cells (V79) were mutagenized with nitrosoguanidine and independent colonies were isolated and expanded. DNA fingerprints were then obtained after digestion with HinfI or HaeIII and hybridization with 33.15 and 33.6 probes (Jeffreys et al., 1985). 12 colonies from untreated cells were also...
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