Article
Detection of recombinational mutations in cultured human cells by Southern blot analysis with minisatellite DNA probes.
Mutation research - 1 Apr 1993
Honma M, Kataoka E, Ohnishi K, Kikuno T, Hayashi M, Sofuni T, Mizusawa H
Abstract excerpt
Using the human acute monocytic leukemia cell line, THP-1, a hypermutability of minisatellite loci was demonstrated in cell culture by Southern blot analysis with minisatellite DNA probes. DNA was isolated from 98 subclones and hybridized to a panel of minisatellite probes consisting of three mul...
Topics
- Base Sequence
- Blotting, Southern
- DNA Fingerprinting
- DNA Probes
- DNA, Neoplasm
- DNA, Satellite
- Humans
- Leukemia, Monocytic, Acute
- Male
- Molecular Sequence Data
- Mutagenicity Tests
- Mutation
- Recombination, Genetic
- Tumor Cells, Cultured