Article
Detection of recombinational mutations in cultured human cells by Southern blot analysis with minisatellite DNA probes.
Mutation research - 1 Apr 1993
Honma M, Kataoka E, Ohnishi K, Kikuno T, Hayashi M, Sofuni T, Mizusawa H
Abstract excerpt
Using the human acute monocytic leukemia cell line, THP-1, a hypermutability of minisatellite loci was demonstrated in cell culture by Southern blot analysis with minisatellite DNA probes. DNA was isolated from 98 subclones and hybridized to a panel of minisatellite probes consisting of three multilocus minisatellite probes (ML probes) and seven locus-specific minisatellite probes (LS probes). The Southern blot...
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