Role of MgATP in the activation and reassociation of cAMP-dependent protein kinase I: consequences of replacing the essential arginine in cAMP binding site A.

doi:10.1021/bi00217a023

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Article

Role of MgATP in the activation and reassociation of cAMP-dependent protein kinase I: consequences of replacing the essential arginine in cAMP binding site A.

Biochemistry - 22 Jan 1991

Neitzel J J, Dostmann W R, Taylor S S

Abstract excerpt

The type I form of cAMP-dependent protein kinase binds MgATP with a high affinity, and binding of MgATP decreases the affinity of the holoenzyme for cAMP [Hofmann et al. (1975) J. Biol. Chem. 250, 7795]. Holoenzyme was formed here with a mutant form of the bovine recombinant type I regulatory sub...

Topics

Adenosine Triphosphate
Arginine
Binding Sites
Cyclic AMP
Enzyme Activation
Escherichia coli
Kinetics
Mutation
Protein Kinases

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