Article
Site-selected insertion of the transposon Tc1 into a Caenorhabditis elegans myosin light chain gene.
Molecular and cellular biology - 1 Feb 1993
Rushforth A M, Saari B, Anderson P
Abstract excerpt
We used the polymerase chain reaction to detect insertions of the transposon Tc1 into mlc-2, one of two Caenorhabditis elegans regulatory myosin light chain genes. Our goals were to develop a general method to identify mutations in any sequenced gene and to establish the phenotype of mlc-2 loss-of-function mutants. The sensitivity of the polymerase chain reaction allowed us to identify nematode populations...
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