Article
Influence of size and polarity of residue 31 in porcine pancreatic phospholipase A2 on catalytic properties.
Protein engineering - 1 Jul 1990
Kuipers O P, Kerver J, van Meersbergen J, Vis R, Dijkman R, Verheij H M, de Haas G H
Abstract excerpt
Residue 31 of porcine pancreatic phospholipase A2 (PLA2) is located at the entrance to the active site. To study the role of residue 31 in PLA2, six mutant enzymes were produced by site-directed mutagenesis, replacing Leu by either Trp, Arg, Ala, Thr, Ser or Gly. Direct binding studies indicated...
Topics
- Amino Acid Sequence
- Animals
- Kinetics
- Micelles
- Molecular Sequence Data
- Mutation
- Pancreas
- Phosphatidylcholines
- Phospholipases A
- Phospholipases A2
- Protein Conformation
- Spectrometry, Fluorescence
- Substrate Specificity
- Swine