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Article

Influence of size and polarity of residue 31 in porcine pancreatic phospholipase A2 on catalytic properties.

Protein engineering - 1 Jul 1990

Kuipers O P, Kerver J, van Meersbergen J, Vis R, Dijkman R, Verheij H M, de Haas G H

Abstract excerpt

Residue 31 of porcine pancreatic phospholipase A2 (PLA2) is located at the entrance to the active site. To study the role of residue 31 in PLA2, six mutant enzymes were produced by site-directed mutagenesis, replacing Leu by either Trp, Arg, Ala, Thr, Ser or Gly. Direct binding studies indicated a three to six times greater affinity of the Trp31 PLA2 for both monomeric and micellar substrate analogs, relative to...

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Identifiers

PMID: 2217133
DOI: 10.1093/protein/3.7.599

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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