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Article

Purification and characterization of giant empty proheads from packaging-defective 23ptg mutants of bacteriophage T4.

Virology - 1 Oct 1993

Rao V B, Leffers G

Abstract excerpt

We constructed packaging-defective gene 23ptg mutants of bacteriophage T4 that produced giant proheads of varying lengths. The giant proheads constituted up to 20% of the total proheads. A purification protocol was developed to prepare large quantities of highly pure giant proheads. The purified giant proheads appeared to package both mature T4 DNA and concatemeric AD10 plasmid DNA in vitro.

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Identifiers

PMID: 8372455
DOI: 10.1006/viro.1993.1554

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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