Article
Purification and characterization of giant empty proheads from packaging-defective 23ptg mutants of bacteriophage T4.
Virology - 1 Oct 1993
Rao V B, Leffers G
Abstract excerpt
We constructed packaging-defective gene 23ptg mutants of bacteriophage T4 that produced giant proheads of varying lengths. The giant proheads constituted up to 20% of the total proheads. A purification protocol was developed to prepare large quantities of highly pure giant proheads. The purified...
Topics
- Bacteriophage T4
- Capsid
- Cloning, Molecular
- Genetic Vectors
- Microscopy, Electron
- Mutation
- Negative Staining
