Length quantization of DNA partially expelled from heads of a bacteriophage T3 mutant.

doi:10.1016/j.virol.2014.03.016

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Article

Length quantization of DNA partially expelled from heads of a bacteriophage T3 mutant.

Virology - 1 May 2014

Serwer Philip, Wright Elena T, Liu Zheng, Jiang Wen

Abstract excerpt

DNA packaging of phages phi29, T3 and T7 sometimes produces incompletely packaged DNA with quantized lengths, based on gel electrophoretic band formation. We discover here a packaging ATPase-free, in vitro model for packaged DNA length quantization. We use directed evolution to isolate a five-site T3 point mutant that hyper-produces tail-free capsids with mature DNA (heads). Three tail gene mutations, but no head...

Topics

Bacteriophage T3
DNA, Viral
Deoxyribonuclease I
Electrophoresis
Microscopy, Electron, Transmission
Mutation
Virion
Virus Assembly

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Identifiers

PMID: 24889235
DOI: 10.1016/j.virol.2014.03.016
PMCID: PMC4044629

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