Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

In vitro packaging of DNA of the Bacillus subtilis bacteriophage SPP1.

Journal of molecular biology - 11 Feb 2000

Dröge A, Tavares P

Abstract excerpt

In vitro packaging of bacteriophage SPP1 DNA into procapsids is described and the requirements of this process were determined. Combination of proheads with an extract supplying terminase, DNA and phage tails yielded up to 10(7 )viable phages per milliliter of in vitro reaction under optimized conditions. The presence of neutral polymers and polyamines had a concentration and type dependent effect in the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacillus Phages
Share to Bacillus subtilis
Share to Capsid

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Endodeoxyribonucleases

Share to Polyamines

Share to Polymers

Share to Protein Precursors

Share to Virus Assembly

Source

Identifiers

PMID: 10656820
DOI: 10.1006/jmbi.1999.3449

Genes

SPP1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

In vitro packaging of DNA of the Bacillus subtilis bacteriophage SPP1.PMID 10656820Read ↗

The portal protein plays essential roles at different steps of the SPP1 DNA packaging process.Semantic proximity Read ↗

Specific interaction of terminase, the DNA packaging enzyme of bacteriophage lambda, with the portal protein of the prohead.Semantic proximity Read ↗

Identification of a gene in Bacillus subtilis bacteriophage SPP1 determining the amount of packaged DNA.Semantic proximity Read ↗

Head morphogenesis genes of the Bacillus subtilis bacteriophage SPP1.Semantic proximity Read ↗

Initial cos cleavage of bacteriophage lambda concatemers requires proheads and gpFI in vivo.Semantic proximity Read ↗

Assembly and maturation of the bacteriophage lambda procapsid: gpC is the viral protease.Semantic proximity Read ↗

Origin and function of the two major tail proteins of bacteriophage SPP1.Semantic proximity Read ↗

Purification and characterization of giant empty proheads from packaging-defective 23ptg mutants of bacteriophage T4.Semantic proximity Read ↗

The nuclease domain of the SPP1 packaging motor coordinates DNA cleavage and encapsidation.Semantic proximity Read ↗

Efficient DNA packaging of bacteriophage PRD1 requires the unique vertex protein P6.Semantic proximity Read ↗

Isolation and analysis of mutants of double-stranded-RNA bacteriophage phi6 with altered packaging specificity.Semantic proximity Read ↗

Bacteriophage p22 portal vertex formation in vivo.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.