Article
Isolation and characterization of the smallest bacteriophage P4 derivatives packaged into P4-size head in bacteriophage P2-P4 system.
Journal of microbiology (Seoul, Korea) - 1 Oct 2006
Kim Kyoung-Jin, Song Jaeho
Abstract excerpt
Bacteriophage P4, a satellite phage of coliphage P2, is a very useful experimental tool for the study of viral capsid assembly and cos-cleavage. For an in vitro cos-cleavage reaction study of the P2-P4 system, new shortened and selectable markers containing P4 derivative plasmids were designed as a substrate molecules. They were constructed by swapping the non-essential segment of P4 DNA for either the kanamycin...
Topics
- Bacteriophage P2
- Capsid
- Coliphages
- DNA Restriction Enzymes
- DNA, Circular
- Genome, Viral
- Hot Temperature
- Mutation
- Virus Assembly
