Article
Generality of the shared active site among yeast family site-specific recombinases. The R site-specific recombinase follows the Flp paradigm [corrected].
The Journal of biological chemistry - 29 Apr 1994
Yang S H, Jayaram M
Abstract excerpt
Mutations of the invariant Int family tetrad residues, the RHR triad, and the active site tyrosine, within the Zygosaccharomyces rouxii site-specific recombinase R cause the same "step-arrest" phenotypes as they do in the Flp recombinase of Saccharomyces cerevisiae. In "half-site" recombinations, the R recombinase exhibits catalytic complementation between an RHR triad mutant and an active site tyrosine mutant....
Topics
- Arginine
- Base Sequence
- Binding Sites
- Catalysis
- DNA Nucleotidyltransferases
- DNA, Fungal
- Genetic Complementation Test
- Histidine
- Hydrolysis
- Molecular Sequence Data
- Mutation