Generality of the shared active site among yeast family site-specific recombinases. The R site-specific recombinase follows the Flp paradigm [corrected].

Abstract excerpt

Mutations of the invariant Int family tetrad residues, the RHR triad, and the active site tyrosine, within the Zygosaccharomyces rouxii site-specific recombinase R cause the same "step-arrest" phenotypes as they do in the Flp recombinase of Saccharomyces cerevisiae. In "half-site" recombinations, the R recombinase exhibits catalytic complementation between an RHR triad mutant and an active site tyrosine mutant....

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• Share to Base Sequence
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