Article
Simultaneous genotyping of sex and kappa-casein of bovine in vitro fertilized embryos by the PCR technique.
Zentralblatt fur Veterinarmedizin. Reihe A - 1 May 1993
Schellander K, Mayr B, Ertl K, Peli J
Abstract excerpt
Sex and the kappa-casein genotype of in vitro produced cattle demiembryos was identified by simultaneous PCR-genotyping using polymorphic sites in ZFY/ZFX and kappa-casein genes. We obtained PCR products of 530 bp (kappa-casein) and of 445/447 bp (sex). Sex and the two kappa-casein alleles could be identified after digestion of the PCR products with Pstl and simultaneous separation of the restriction fragments on...
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