Article
Sexing and multiple genotype analysis from a single cell of bovine embryo.
Theriogenology - 15 Mar 2001
Chrenek P, Boulanger L, Heyman Y, Uhrin P, Laurincik J, Bulla J, Renard J P
Abstract excerpt
We described a procedure for multiple genotype analysis (determination of sex and of three genetic markers) from a single cell derived from bovine preimplantation embryo. It consists of primer extension preamplification-polymerase chain reaction (PEP-PCR) and subsequent single assay or multiplex PCR. A single blastomere that was isolated by microaspiration from bovine embryos at the 16- to 32-cell stage then was...
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