Article
Technical note: Detection of bovine kappa-casein variants A, B, C, and E by means of polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP).
Journal of animal science - 1 Jun 1998
Barroso A, Dunner S, Cañón J
Abstract excerpt
We used polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis to screen the most frequent variants (A, B, C, and E) found in the bovine kappa-casein gene. The PCR products (453 bp) were heat-denatured, loaded onto nondenaturing polyacrylamide gels, and silver-stain...
Topics
- Alleles
- Animals
- Breeding
- Caseins
- Cattle
- Cost-Benefit Analysis
- DNA
- Genetic Variation
- Genotype
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single-Stranded Conformational
