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Article

Site-directed mutations in the relaxase operon of RP4.

Journal of bacteriology - 1 Aug 1993

Cole S P, Lanka E, Guiney D G

Abstract excerpt

Mutations were constructed by site-directed mutagenesis in the relaxase operon of the broad-host-range plasmid RP4. The mutations were constructed in smaller plasmids, recombined into the 60-kb RP4 plasmid, and tested for their ability to transfer. The relaxase operon contains the transfer genes...

Topics

Bacterial Proteins
Conjugation, Genetic
Gram-Negative Bacteria
Mutagenesis, Site-Directed
Operon
Phenotype
Plasmids
Recombination, Genetic
Transfection

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 8335645
DOI: 10.1128/jb.175.15.4911-4916.1993
PMCID: PMC204946

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8335645-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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