Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Site-directed mutations in the relaxase operon of RP4.

Journal of bacteriology - 1 Aug 1993

Cole S P, Lanka E, Guiney D G

Abstract excerpt

Mutations were constructed by site-directed mutagenesis in the relaxase operon of the broad-host-range plasmid RP4. The mutations were constructed in smaller plasmids, recombined into the 60-kb RP4 plasmid, and tested for their ability to transfer. The relaxase operon contains the transfer genes traJ, traH, and traI, which are involved in nicking at the transfer origin to generate the single strand destined to be...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Proteins
Share to Conjugation, Genetic
Share to Mutagenesis, Site-Directed

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Operon

Share to Phenotype

Share to Plasmids

Share to Recombination, Genetic

Share to Transfection

Source

Open in PubMed Open full text

Identifiers

PMID: 8335645
DOI: 10.1128/jb.175.15.4911-4916.1993
PMCID: PMC204946

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Site-directed mutations in the relaxase operon of RP4.PMID 8335645Read ↗

Essential motifs of relaxase (TraI) and TraG proteins involved in conjugative transfer of plasmid RP4.Semantic proximity Read ↗

Hin-mediated DNA knotting and recombining promote replicon dysfunction and mutation.Semantic proximity Read ↗

F plasmid conjugative DNA transfer: the TraI helicase activity is essential for DNA strand transfer.Semantic proximity Read ↗

Functional and mutational analysis of conjugative transfer region 2 (Tra2) from the IncHI1 plasmid R27.Semantic proximity Read ↗

Discovery of a new family of relaxases in Firmicutes bacteriaSemantic proximity Read ↗

Tyrosine partners coordinate DNA nicking by the Salmonella typhimurium plasmid pCU1 relaxase enzyme.Semantic proximity Read ↗

TraY and integration host factor oriT binding sites and F conjugal transfer: sequence variations, but not altered spacing, are tolerated.Semantic proximity Read ↗

MobB protein stimulates nicking at the R1162 origin of transfer by increasing the proportion of complexed plasmid DNA.Semantic proximity Read ↗

A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation.Semantic proximity Read ↗

Modifying bacteriophage lambda with recombineering.Semantic proximity Read ↗

Interaction between the RP4 coupling protein TraG and the pBHR1 mobilization protein MobSemantic proximity Read ↗

Identification and characterization of RP1 Tra1 cistrons involved in pilus function and plasmid mobilization.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.