Identification and characterization of RP1 Tra1 cistrons involved in pilus function and plasmid mobilization.

doi:10.1128/jb.175.2.448-456.1993

Back to search

Article

Identification and characterization of RP1 Tra1 cistrons involved in pilus function and plasmid mobilization.

Journal of bacteriology - 1 Jan 1993

Fong S T, Stanisich V A

Abstract excerpt

Transfer-defective mutants of the Tra1 region of RP1 were isolated. Complementation studies involving stable heterozygotes combined with the mapping of Tn5 insertion mutations revealed two pilus cistrons, pilA and pilB, at positions 46.9 to 48.2 kb and 46.0 to 46.4 kb, respectively. All pilB muta...

Topics

Alleles
Cloning, Molecular
Conjugation, Genetic
DNA, Bacterial
Escherichia coli
Fimbriae, Bacterial
Genes, Bacterial
Genetic Complementation Test
Genotype
Heterozygote
Mutagenesis
Mutagens
Plasmids
Pseudomonas aeruginosa
Restriction Mapping

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.