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Article

Plasmid transformation of Streptomyces venezuelae: modified procedures used to introduce the gene(s) for p-aminobenzoate synthase.

Journal of general microbiology - 1 Apr 1990

Aidoo D A, Barrett K, Vining L C

Abstract excerpt

Sucrose was unsuitable as an osmotic stabilizer in buffer solutions and media used for transformation of Streptomyces venezuelae ISP5230. Its replacement with NaCl, together with other modifications in the procedure, allowed efficient formation and regeneration of protoplasts but did not support transformation of S. venezuelae ISP5230 by vectors pIJ41 and pIJ941. With pIJ702, transformants with a low...

Topics

4-Aminobenzoic Acid
Chloramphenicol
DNA, Bacterial
Genes, Bacterial
Mutation
Plasmids
Protoplasts
Restriction Mapping
Streptomyces
Sucrose
Transaminases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Transformation, Bacterial

Source

Identifiers

PMID: 2398345
DOI: 10.1099/00221287-136-4-657

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2398345-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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