Article
[Transposition of Tn5096 in a agricultural antibiotic 120 roducer Streptomyces hygrospinocus var. beijingensis RF220].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Dec 1999
Zhang Y, Zhu C, Bai L, Wang Y
Abstract excerpt
No transformant was obtained when pCZA168(bla, tsr, Tn5096, ColEI rep. Strep repts) was used to transform S. hygropinocus RF220. pIJ702 isolated from S. hygroscopicus N103 was transformed into RF220 at a low frequency. pIJ702 plasmid was cured in RF220 transformant and it was re-transformed into its cured FR220 strain, but the transformation frequency was not increased significantly, suggesting that...
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