Article
[MRSA--detection of mecA and its regulatory genes].
Rinsho byori. The Japanese journal of clinical pathology - 1 Nov 1993
Kagawa S, Yamashita K, Matsuoka A
Abstract excerpt
Methicillin resistance in S.aureus and S.epidermidis strains is primarily due to production of a new penicillin-binding protein PBP2' with extremely low binding affinity to most beta-lactam antibiotics. The structural gene for PBP2', mecA, is detectable in clinical specimens by using the polymerase chain reaction (PCR). Amplified target DNA of 630bp can be resolved on ethidium bromide-stained gels, and hybridized...
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