Article
Expression of a Drosophila GABA receptor in a baculovirus insect cell system. Functional expression of insecticide susceptible and resistant GABA receptors from the cyclodiene resistance gene Rdl.
FEBS letters - 13 Dec 1993
Lee H J, Rocheleau T, Zhang H G, Jackson M B, ffrench-Constant R H
Abstract excerpt
Recombinant baculoviruses containing two alternative splice forms of the Drosophila Rdl GABA receptor gene were constructed. Spodoptera frugiperda (Sf21) cells infected with either splice form expressed a transcript of expected size (2.5 kb). Western blotting of cell membrane extracts and immunop...
Topics
- Alternative Splicing
- Animals
- Baculoviridae
- Cell Line
- Cloning, Molecular
- Drosophila
- Drosophila Proteins
- Insecticide Resistance
- Membrane Potentials
- Moths
- Mutation
- Picrotoxin
- Receptors, GABA-A