Article
Direct, automated detection of rifampin-resistant Mycobacterium tuberculosis by polymerase chain reaction and single-strand conformation polymorphism analysis.
Antimicrobial agents and chemotherapy - 1 Oct 1993
Telenti A, Imboden P, Marchesi F, Schmidheini T, Bodmer T
Abstract excerpt
A rapid screening test was recently established for the detection of mutations in the rpoB gene of Mycobacterium tuberculosis, a region identified as the locus for rifampin resistance (Rifr). The detection method involved the amplification by polymerase chain reaction (PCR) of the Rifr region and...
Topics
- Base Sequence
- DNA, Single-Stranded
- Drug Resistance, Microbial
- Evaluation Studies as Topic
- Humans
- Molecular Sequence Data
- Mutation
- Mycobacterium tuberculosis
- Nucleic Acid Conformation
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Rifampin
- Sputum