Article
Crystal structure of the bromide-bound D85S mutant of bacteriorhodopsin: principles of ion pumping.
Biophysical journal - 1 Jul 2003
Facciotti Marc T, Cheung Vincent S, Nguyen Doris, Rouhani Shahab, Glaeser Robert M
Abstract excerpt
We report the crystal structure of a bromide-bound form of the D85S mutant of bacteriorhodopsin, bR(D85S), a protein that uses light energy rather than ATP to pump halide ions across the cell membrane. Comparison of the structure of the halide-bound and halide-free states reveals that both displacements of individual side-chain positions and concerted helical movements occur on the extracellular side of the...
Topics
- Bacteriorhodopsins
- Binding Sites
- Bromides
- Computer Simulation
- Crystallography, X-Ray
- Ion Pumps
- Models, Chemical
- Models, Molecular
- Molecular Conformation
- Mutation
- Protein Binding
- Protein Conformation
- Protein Structure, Tertiary
- Solvents