Article
Evidence for orientation-conserved transfer in the TCA cycle in Saccharomyces cerevisiae: 13C NMR studies.
Biochemistry - 30 Nov 1993
Sumegi B, Sherry A D, Malloy C R, Srere P A
Abstract excerpt
When [4-13C]glutamate is incubated with yeast cells, the [13C]aspartate formed shows a [2-13C]/[3-13C] ratio that is greater than 1. If the conversion occurs through the Krebs TCA cycle with the symmetrical intermediates succinate and fumarate, the ratio is expected to be 1. The addition of fluoroacetate to the incubation increases the ratio further. When the mutants Aco1- or CS1- are used, little or no aspartate...
Topics
- Acetates
- Acyl Coenzyme A
- Aspartic Acid
- Carbon Isotopes
- Citric Acid Cycle
- Glutamates
- Magnetic Resonance Spectroscopy
- Models, Biological
- Mutation
- Saccharomyces cerevisiae