Article
A multinuclear magnetic resonance study of a cls11 mutant showing the Pet- phenotype of Saccharomyces cerevisiae.
European journal of biochemistry - 5 Oct 1990
Galons J P, Tanida I, Ohya Y, Anraku Y, Arata Y
Abstract excerpt
Energetic and intermediary metabolism was studied in a Pet- mutant of Saccharomyces cerevisiae with a calcium-sensitive phenotype that shows an inability to grow when cultured in a medium containing non-fermentable substrates. The perchloric acid extracts were prepared from suspensions of cls11 mutant and wild-type cells incubated with [1,3-13C]glycerol or [2-13]acetate, and analyzed by 31P, 13C and 1H NMR. 31P-...
Topics
- Acetates
- Amino Acids
- Carbohydrate Metabolism
- Citric Acid Cycle
- Glycerol
- Glyoxylates
- Magnetic Resonance Spectroscopy
- Mutation
- Nucleotides
- Phosphates
- Pyruvate Decarboxylase
