Article
ECGF and heparin determine differentiation of cloned cerebral endothelial cells in vitro.
Molecular and chemical neuropathology - 1 Sept 1993
Amberger A, Lemkin P F, Sonderegger P, Bauer H C
Abstract excerpt
Protein expression patterns of morphologically different cloned capillary endothelial cells from porcine and murine brain cortices were examined. Type I cells, grown in medium containing heparin and endothelial cell growth factor (ECGF), exhibited a polygonal, cobblestone appearance and appeared to replicate the cells of the blood-brain barrier endothelium. Type II cells, grown in medium without heparin and ECGF,...
Topics
- Actins
- Animals
- Blotting, Northern
- Brain
- Cell Differentiation
- Cells, Cultured
- Clone Cells
- Drug Synergism
- Electrophoresis, Polyacrylamide Gel
- Endothelial Growth Factors
- Endothelium, Vascular