Article
Isolation and culture of microvascular endothelial cells from gliomas of different WHO grades.
Journal of neuro-oncology - 1 Jan 2006
Miebach Sabine, Grau Stefan, Hummel Vera, Rieckmann Peter, Tonn Joerg-Christian, Goldbrunner Roland Helmut
Abstract excerpt
Gliomas are the most common intrinsic brain tumors. The degree of vascularization corresponds to malignancy and is related to prognosis. In order to retrieve information about tumor behavior in situ, the use of primary tissue material for experiments is advantageous. With increasing evidence for the importance of microenvironment and vascularization in tumor biology, we concentrated on the isolation of...
Topics
- Brain Neoplasms
- Cell Culture Techniques
- Cell Survival
- Endothelial Cells
- Glioma
- Humans
- Immunomagnetic Separation
- Neovascularization, Pathologic
- Phenotype
- Reproducibility of Results