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Article

Role of the physical state of Salmonella lipopolysaccharide in expression of biological and endotoxic properties.

Infection and immunity - 1 Dec 1993

Shnyra A, Hultenby K, Lindberg A A

Abstract excerpt

Lipopolysaccharide (LPS) extracted from three strains of Salmonella typhimurium, i.e., the rough Re mutant SL1102, the rough Ra mutant TV119, and the smooth strain SH4809, was first electrodialyzed (eLPS) and then divalent cation deprived by EDTA treatment and finally made monomeric by deoxycholate solubilization. The removal of excess detergent by extensive dialysis in the absence of mineral cations resulted in...

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Identifiers

PMID: 8225609
DOI: 10.1128/iai.61.12.5351-5360.1993
PMCID: PMC281322

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