Article
Isolation of the TYE2 gene reveals its identity to SWI3 encoding a general transcription factor in Saccharomyces cerevisiae.
Current genetics - 1 Sept 1993
Löhning C, Rosenbaum C, Ciriacy M
Abstract excerpt
The TYE2 gene was identified by recessive mutations which result in a significant reduction of Ty-mediated ADH2 expression. We cloned the TYE2 gene and analyzed its sequence. A large open reading frame of 825 codons was found encoding a rather hydrophilic, 93-kilodalton protein which contains a highly acidic region at its N-terminus. By sequence comparison we found that TYE2 is identical to gene SWI3 which has...
Topics
- Blotting, Northern
- Cloning, Molecular
- Fungal Proteins
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- Open Reading Frames
- Phenotype
- Restriction Mapping
- Saccharomyces cerevisiae