Article
Use of hybridization for distance measurement by fluorescence energy transfer in oligomeric proteins: distance between two functional sites in aspartase.
Biochemical and biophysical research communications - 30 Sept 1993
Murase S, Kawata Y, Yumoto N
Abstract excerpt
Our previous studies suggested that in a tetrameric enzyme aspartase, Cys-140 and Trp-430 are located at or near the catalytic and activator sites, respectively. To estimate the distance between these two sites, fluorescence energy transfer between a single tryptophan (Trp-430) and a fluorescent...
Topics
- Aspartate Ammonia-Lyase
- Binding Sites
- Cysteine
- Energy Transfer
- Fluorescent Dyes
- Maleimides
- Models, Chemical
- Mutation
- Protein Conformation
- Spectrometry, Fluorescence
- Sulfhydryl Reagents
- Tryptophan