Article
Compact thermally-denatured state of a staphylococcal nuclease mutant from resonance energy transfer measurements.
Biophysical chemistry - 1 Dec 1993
Wu P G, James E, Brand L
Abstract excerpt
Thermal denaturation of a staphylococcal nuclease mutant K78C, where lysine 78 is replaced by cysteine, was studied by circular dichroism (CD) and resonance energy transfer. CD spectra suggest that residual structures remain in the denatured state. Steady-state energy transfer from intrinsic tyro...
Topics
- Chemical Phenomena
- Chemistry, Physical
- Circular Dichroism
- Fluorescent Dyes
- Heating
- Micrococcal Nuclease
- Mutation
- Naphthalenesulfonates
- Protein Conformation
- Protein Denaturation
- Protein Structure, Tertiary
- Spectrometry, Fluorescence
- Thermodynamics
- Tryptophan
- Tyrosine