Article
Purification and characterization of fatty acid synthase from Candida albicans strain 4918 and two derived spontaneous cerulenin-resistant mutants.
Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology - 1 Jan 1994
McElhaney-Feser G E, Cihlar R L
Abstract excerpt
Fatty acid synthase from three strains of Candida albicans (parental strain 4918, and two spontaneous cerulenin-resistant mutants, 4918-2 and 4918-10) has been purified and characterized. In all three cases the purification protocol included ammonium sulfate precipitation, fractionation with butyl-Toyopearl, differential centrifugation and sedimentation velocity centrifugation. Inclusion of protease inhibitors,...
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