Article
Synthesis, cloning and sequencing of glucoamylase cDNA from Aspergillus niger mutant T21.
Chinese journal of biotechnology - 1 Jan 1993
Tang G, Xu Y, Gong H, Zhong L, Yang K
Abstract excerpt
Poly(A)+ RNA was isolated from Aspergillus niger T21, a glucoamylase overproducing strain and was used as template to synthesize double stranded cDNA. A cDNA library was then constructed. The E. coli transformants were screened for the glucoamylase cDNA by in situ colony hybridization with P-labeled fragment of glucoamylase gene as probe. The positive rate was 1.6%. Restriction analysis proved that 32% of the...
Topics
- Aspergillus niger
- Base Sequence
- Cloning, Molecular
- DNA, Complementary
- DNA, Fungal
- Glucan 1,4-alpha-Glucosidase
- Mutation
- Poly A
- RNA, Fungal