Article
Synthesis, cloning and sequencing of glucoamylase cDNA from Aspergillus niger mutant T21.
Chinese journal of biotechnology - 1 Jan 1993
Tang G, Xu Y, Gong H, Zhong L, Yang K
Abstract excerpt
Poly(A)+ RNA was isolated from Aspergillus niger T21, a glucoamylase overproducing strain and was used as template to synthesize double stranded cDNA. A cDNA library was then constructed. The E. coli transformants were screened for the glucoamylase cDNA by in situ colony hybridization with P-labeled fragment of glucoamylase gene as probe. The positive rate was 1.6%. Restriction analysis proved that 32% of the...
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