Article
Bimutation breeding of Aspergillus niger strain for enhancing β-mannanase production by solid-state fermentation.
Carbohydrate research - 18 Oct 2011
Wu Minchen, Tang Cunduo, Li Jianfang, Zhang Huimin, Guo Jing
Abstract excerpt
A parent strain Aspergillus niger LW-1 was mutated by the compound mutagenesis of vacuum microwave (VMW) and ethyl methane sulfonate (EMS). A mutant strain, designated as A. niger E-30, with high- and stable-yield β-mannanase was obtained through a series of screening. The β-mannanase activity of the mutant strain E-30, cultivated on the basic fermentation medium at 32°C for 96 h, reached 36,675 U/g dried koji,...
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