Article
Platelet-mediated transformation of mtDNA-less human cells: analysis of phenotypic variability among clones from normal individuals--and complementation behavior of the tRNALys mutation causing myoclonic epilepsy and ragged red fibers.
American journal of human genetics - 1 Jun 1994
Chomyn A, Lai S T, Shakeley R, Bresolin N, Scarlato G, Attardi G
Abstract excerpt
In the present work, we demonstrate the possibility of using human blood platelets as mitochondrial donors for the repopulation of mtDNA-less (rho 0) cells. The noninvasive nature of platelet isolation, combined with the prolonged viability of platelet mitochondria and the simplicity and efficiency of the mitochondria-transfer procedure, has substantially increased the applicability of the rho 0 cell...
Topics
- Adult
- Aged
- Blood Platelets
- Cell Fusion
- Cell Line
- DNA Mutational Analysis
- DNA, Mitochondrial
- Electron Transport Complex IV
- Female
- Genetic Complementation Test