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Article

Screening human genes for small alterations performing an enzymatic cleavage mismatched analysis (ECMA) protocol.

Molecular biotechnology - 1 Sept 2013

Vogiatzakis Nikolaos, Kekou Kyriaki, Sophocleous Christalena, Kitsiou Sophia, Mavrou Ariadni, Bakoula Chrisa, Kanavakis Emmanouel

Abstract excerpt

Many human diseases are caused by small alterations in the genes and in the majority of cases sophisticated protocols are required for their detection. In this study we estimated the efficacy of an enzymatic protocol, which using a new mismatch-specific DNA plant endonuclease from celery (CEL family) recognizes and cleaves mismatched alleles between mutant and normal PCR products. The protocol was standardized on...

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Source

Identifiers

PMID: 23934597
DOI: 10.1007/s12033-007-0062-9

Genes

DMD
CEL

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

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Screening human genes for small alterations performing an enzymatic cleavage mismatched analysis (ECMA) protocol.PMID 23934597Read ↗

Development of a simple and highly sensitive mutation screening system by enzyme mismatch cleavage with optimized conditions for standard laboratories.Semantic proximity Read ↗

Enzymatic mutation detection technologies.Semantic proximity Read ↗

Current methods of mutation detection.Semantic proximity Read ↗

SNP genotyping by heteroduplex analysis.Semantic proximity Read ↗

Fluorescent chemical cleavage of mismatches for efficient screening of the factor VIII gene.Semantic proximity Read ↗

Applying and testing the conveniently optimized enzyme mismatch cleavage method to clinical DNA diagnosis.Semantic proximity Read ↗

PCR-based method to create a specific mutation as a reference for oligomelting.Semantic proximity Read ↗

Simple, rapid detection of PCR heteroduplexes in DNA mutations and polymorphisms.Semantic proximity Read ↗

Mutation detection by denaturing gradient gel electrophoresis (DGGE)Semantic proximity Read ↗

Enzymatic and chemical cleavage methods.Semantic proximity Read ↗

Improved strategy for mutation detection--a modification to the enzyme mismatch cleavage method.Semantic proximity Read ↗

A multiplex single nucleotide polymorphism genotyping method using ligase-based mismatch discrimination and CE-SSCP.Semantic proximity Read ↗

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