Article
A human protein containing a "cold shock" domain binds specifically to H-DNA upstream from the human gamma-globin genes.
The Journal of biological chemistry - 13 May 1994
Horwitz E M, Maloney K A, Ley T J
Abstract excerpt
We previously determined that a region between positions -228 and -189 upstream from the human gamma-globin genes can form an intramolecular triplex (H-DNA) in supercoiled plasmids. To identify proteins that might interact with this DNA structure, we performed expression cloning using an adult bo...
Topics
- Adult
- Base Sequence
- Binding Sites
- Chromatography, Affinity
- DNA, Superhelical
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Globins
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plasmids
- Promoter Regions, Genetic
- Protein Structure, Secondary
- Pyrimidines
- Recombinant Proteins