Article
Suppression of a temperature-sensitive cdc33 mutation of yeast by a multicopy plasmid expressing a Drosophila ribosomal protein.
The Journal of biological chemistry - 20 May 1994
Lavoie C, Tam R, Clark M, Lee H, Sonenberg N, Lasko P
Abstract excerpt
The Saccharomyces cerevisiae cdc33ts4-2 mutant produces a temperature-sensitive allele of the cap-binding subunit of eukaryotic initiation factor-4F (also termed eIF-4E). From a Drosophila cDNA library constructed in a multicopy yeast shuttle vector, a clone was isolated which restored the ability to grow at elevated temperature to cdc33ts4-2 cells. The rescuing Drosophila clone encodes a small ribosomal subunit...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Brassica
- DNA
- Drosophila melanogaster
- Eukaryotic Initiation Factor-4E
- Genes, Fungal
- Molecular Sequence Data