Article
Cloning of the STE5 gene of Saccharomyces cerevisiae as a suppressor of the mating defect of cdc25 temperature-sensitive mutants.
Proceedings of the National Academy of Sciences of the United States of America - 15 Jun 1993
Perlman R, Yablonski D, Simchen G, Levitzki A
Abstract excerpt
The STE5 gene of Saccharomyces cerevisiae was cloned using a screening procedure designed to isolate genes of the S. cerevisiae pheromone response pathway. We screened a yeast genomic high-copy-number plasmid library for genes that allow mating of cdc25ts mutants at the restrictive temperature wi...
Topics
- Adaptor Proteins, Signal Transducing
- Amino Acid Sequence
- Base Sequence
- Carrier Proteins
- Cell Cycle Proteins
- Cloning, Molecular
- Fungal Proteins
- Gene Deletion
- Gene Expression
- Genes, Fungal
- Genes, Mating Type, Fungal
- Genes, Suppressor
- Genotype
- Mating Factor
- Molecular Sequence Data
- Peptides
- Pheromones
- RNA, Messenger