Article
Transgenic cattle resulting from biopsied embryos: expression of c-ski in a transgenic calf.
Biology of reproduction - 1 Mar 1994
Bowen R A, Reed M L, Schnieke A, Seidel G E, Stacey A, Thomas W K, Kajikawa O
Abstract excerpt
Producing transgenic cattle by microinjection of DNA into pronuclei has been inefficient and costly, in large part because of the cost of maintaining numerous nontransgenic pregnancies to term. We designed a system for early identification of transgenic embryos in which biopsies of embryos were assayed by polymerase chain reaction for presence of the transgene before embryo transfer. A total of 2555 embryos were...
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