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Efficient Production of Transgenic Cloned Calves Using Preimplantation Screening

1 Nov 2002

Abstract excerpt

The genetic manipulation of donor cells before nuclear transfer (NT) enables prior selection for transgene integration. However, selection for genetically modified cells using antibiotic drugs often results in mixed populations, resulting in a mixture of transgenic and nontransgenic donor cells for NT. In this study, we attempted to develop efficient strategies for the generation of human bile salt-stimulated...

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PMID: 12390880
DOI: 10.1095/biolreprod.102.006981

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