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Article

Genetic instability of vaccinia virus containing artificially duplicated genome regions.

Virus research - 1 Jan 1994

Kriajevska M V, Zakharova L G, Altstein A D

Abstract excerpt

A double recombinant of vaccinia virus (W-lacZ/J-tk/F) was obtained, which contains two inverted copies of the virus tk gene, separated by 45 kb: (i) the native copy located in the HindIII J fragment of the virus genome was inactivated due to insertion of E. coli lacZ gene; (ii) the second active copy was artificially inserted into the HindIII F fragment. The virus expressing both thymidine kinase and...

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PMID: 8165864
DOI: 10.1016/0168-1702(94)90075-2

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