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Article

Structure-function relationship of arginyl-tRNA synthetase from Escherichia coli: isolation and characterization of the argS mutation MA5002.

Nucleic acids research - 25 Mar 1990

Eriani G, Dirheimer G, Gangloff J

Abstract excerpt

The Escherichia coli K12 argS MA5002 mutant appears to have a functionally altered arginyl-tRNA synthetase (ArgRS). The gene coding for this enzyme was isolated from E. coli genomic DNA using the PCR procedure and inserted into a pUC18 multicopy vector. Sequencing revealed that it differs from the wildtype ArgRS structural gene only by one mutation: a replacement of a C by an A residue which results in...

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Identifiers

PMID: 2183195
DOI: 10.1093/nar/18.6.1475
PMCID: PMC330514

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