Article
Structure-function relationship of arginyl-tRNA synthetase from Escherichia coli: isolation and characterization of the argS mutation MA5002.
Nucleic acids research - 25 Mar 1990
Eriani G, Dirheimer G, Gangloff J
Abstract excerpt
The Escherichia coli K12 argS MA5002 mutant appears to have a functionally altered arginyl-tRNA synthetase (ArgRS). The gene coding for this enzyme was isolated from E. coli genomic DNA using the PCR procedure and inserted into a pUC18 multicopy vector. Sequencing revealed that it differs from the wildtype ArgRS structural gene only by one mutation: a replacement of a C by an A residue which results in...
Topics
- Amino Acid Sequence
- Amino Acyl-tRNA Synthetases
- Arginine-tRNA Ligase
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Kinetics
- Molecular Sequence Data
- Mutation