Article
Generation of a mouse strain that produces immunoglobulin kappa chains with human constant regions.
Science (New York, N.Y.) - 19 Nov 1993
Zou Y R, Gu H, Rajewsky K
Abstract excerpt
Humanized antibodies are highly efficient as immunotherapeutic reagents and have many advantages over rodent antibodies. A mouse strain was generated by gene targeting to replace the mouse kappa light chain constant (C) region gene with the human C kappa gene. Mice homozygous for the replacement...
Topics
- Amino Acid Sequence
- Animals
- B-Lymphocytes
- Base Sequence
- Gene Rearrangement
- Genes, Immunoglobulin
- Humans
- Immunoglobulin Constant Regions
- Immunoglobulin Isotypes
- Immunoglobulin kappa-Chains
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Recombinant Fusion Proteins
- Stem Cells
- Transfection