Article
Identification of a putative metal binding site in a new family of metalloregulatory proteins.
The Journal of biological chemistry - 5 Aug 1994
Shi W, Wu J, Rosen B P
Abstract excerpt
The transcription of the ars operon is negatively controlled by the ArsR repressor and induced by arsenite and antimonite. Using hydroxylamine mutagenesis, four arsR mutants were isolated; three were selected for inability to respond to inducers: C32Y, C32F, and C34Y. Each of the three altered proteins still bound specifically to the ars operator, but inducers were less effective in effecting the release of the...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- Cysteine
- DNA
- DNA-Binding Proteins
- Metals
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Trans-Activators