Article
Initiation and termination of DNA transfer at F plasmid oriT.
Molecular microbiology - 1 Feb 1994
Gao Q, Luo Y, Deonier R C
Abstract excerpt
DNA sequences within the F plasmid transfer origin (oriT) were tested for their ability to initiate or terminate conjugal transfer. Mutant and wild-type oriT elements were cloned as direct repetitions flanking the rpsL gene on a pBR322-based plasmid, and the frequency of deletion of this segment during matings sponsored by F'lac (F42) with streptomycin-resistant recipients was measured. Shortened oriT elements...
Topics
- Base Sequence
- Conjugation, Genetic
- DNA, Bacterial
- F Factor
- Molecular Sequence Data
- Mutation
- Sequence Deletion