Article
Optimal infectivity in vitro of human immunodeficiency virus type 1 requires an intact nef gene.
Journal of virology - 1 May 1994
Chowers M Y, Spina C A, Kwoh T J, Fitch N J, Richman D D, Guatelli J C
Abstract excerpt
The replication competence of human immunodeficiency virus type 1 genomes containing mutations in the nef open reading frame was evaluated in continuous cell lines. Mutants that contained a deletion in the nef open reading frame, premature termination codons, or missense mutations in the N-terminal myristoylation signal were constructed. The replication of these mutants was tested in three ways. First, plasmid...
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Fusion
- DNA, Viral
- Genes, nef
- HIV Core Protein p24
- HIV-1
- Hematopoietic Stem Cells
- Humans
- Molecular Sequence Data
- Mutation