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Article

Improved PCR sensitivity for direct genotyping of Chlamydia trachomatis serovars by using a nested PCR.

Journal of clinical microbiology - 1 Feb 1994

Lan J, Ossewaarde J M, Walboomers J M, Meijer C J, van den Brule A J

Abstract excerpt

Successful amplification of omp1 DNA by PCR is crucial in the genotyping of Chlamydia trachomatis when directly performed with clinical samples (J. Lan, J. M. M. Walboomers, R. Roosendaal, G. J. van Doornum, D. M. McLaren, C. J. L. M. Meijer, and A. J. C. van den Brule, J. Clin. Microbiol. 31:1060-1065, 1993). Several primers flanking the four variable domains of the omp1 gene were selected and tested for...

Topics

Bacterial Outer Membrane Proteins
Bacterial Typing Techniques
Base Sequence
Chlamydia Infections
Chlamydia trachomatis
DNA Primers
Female
Genes, Bacterial
Genotype
Humans
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymerase Chain Reaction

Porins

Sensitivity and Specificity

Serotyping

Source

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Identifiers

PMID: 8150967
DOI: 10.1128/jcm.32.2.528-530.1994
PMCID: PMC263068

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8150967-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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