Article
Development of real-time PCR assays for genotyping of Chlamydia trachomatis.
Journal of clinical microbiology - 1 Aug 2007
Jalal Hamid, Stephen Hannah, Alexander Sarah, Carne Christopher, Sonnex Christopher
Abstract excerpt
We have developed and validated a nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of lymphogranuloma venereum (LGV). The 11 probes used in the NRT-PCR correctly identified the DNA from D to K and LGV reference strains and did not cross-react with the DNA from 26 strains representing the...
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