Article
High-resolution HLA-DPB typing based upon computerized analysis of data obtained by fluorescent sequencing of the amplified polymorphic exon 2.
Human immunology - 1 Dec 1993
Versluis L F, Rozemuller E, Tonks S, Marsh S G, Bouwens A G, Bodmer J G, Tilanus M G
Abstract excerpt
To differentiate 32 HLA-DPB alleles, conventional techniques such as serology and cellular typing are inadequate for high-resolution DPB typing. The most refined DNA typing until now is SSO typing and new selected oligonucleotides can be added to this system to distinguish new allele sequences. D...
Topics
- Base Sequence
- Exons
- Fluorescent Dyes
- Genotype
- HLA-DP Antigens
- HLA-DP beta-Chains
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic